How much ethidium bromide for gel

Stir briefly. After reacting for at least 20 hours, adjust the pH to with sodium bicarbonate, then pour the solution down the laboratory sink with a strong stream of running water. Waste Disposal in Academic Institutions.

Lewis Publishers. Add 10mL of fresh bleach for every 1mg EtBr. Stir at room temperature for at least 4 hours. The EtBr is converted to the physiologically inactive product 2-carboxybenzophenone. The solution can then be poured down the laboratory sink with a strong stream of running water.

Solid waste including contaminated pipet tips, gloves, destaining bags, and all used gels are to be deposited in the designated yellow metal waste container in RNS Follow the instructions on the waste container. Needles, scalpels, Pasteur pipettes and other sharps contaminated with EtBr should be disposed of directly into a sharps container. Volumetric or transfer pipettes, and other disposable glassware contaminated with EtBr should be disposed of in the designated glass disposal container.

Grossly contaminated visibly contaminated glassware should be washed with bleach before disposal in the designated container. Test tubes and centrifuge tubes contaminated with EtBr should first be emptied, with the liquid disposed of according to the procedures given above.

Empty tubes can then be disposed of in the trash. Grossly contaminated visibly contaminated tubes should be washed with bleach prior to disposal. Most other disposable labware e. Grossly contaminated visibly contaminated disposable labware should be washed with bleach prior to disposal.

Microwave your gel for however long it takes to melt completely. Be careful not to burn yourself, because it will be hot. Next add ethidium bromide -- this is a chemical that intercalates DNA and makes it visible under UV light.

You might want to wear gloves. The amount of EtBr to add is as follows: of a 0. The casting trays we have have this rubber stuff around the edges that makes a tight seal when you put them in the gel boxes sideways. They also have notches where you can insert a comb. The size comb to use depends on the width you want your wells to be. Set Starty point and interval for your subscription and submit order! By the way: Through your account you can customize or delete your subscriptions anytime. Product details.

Ethidium bromide. Directions for use. Technical Information. Order form. Selected quantity: 0. Order No. German English French. You can search for and download your certificate of analysis for the selected product here. Please provide your batch number. The following analysis certificates have been found:. No certificate of analysis was found for the selected product. To make a request, please provide your e-mail address and the required language. Your request has been sent. Order number:.